Table 1 List of reagents
Name | Source | Type | Host species | Cat. No | Lot No | Dilution |
|---|---|---|---|---|---|---|
Antibodies | ||||||
α-tubulin (TU-02) | SCBT | Monoclonal | Mouse | Sc-8035 | L1416 | 1:1000 |
CAMKK2(ZZ9) | SCBT | Monoclonal | Mouse | Sc-100364 | A0220 | 1:1000 |
GAPDH | SCBT | Monoclonal | Mouse | Sc-25778 | C0910 | 1:1000 |
OXPHOS-cocktail | Abcam | Monoclonal | Mouse | MS601 | P9552 | 1:2000 |
NDUFB8 (CI) | Abcam | Monoclonal | Mouse | ab110242 | NA | NA |
SDHB (CII) | Abcam | Monoclonal | Mouse | ab14714 | NA | NA |
UQCRC2 (CIII) | Abcam | Monoclonal | Mouse | ab14745 | NA | NA |
COXII (CIV) | Abcam | Monoclonal | Mouse | ab110258 | NA | NA |
ATP5A (CV) | Abcam | Monoclonal | Mouse | ab14748 | NA | NA |
SDHA | Abcam | Monoclonal | Rabbit | AB137040 | GR3252256-5 | 1:1000 |
SDHA | SCBT | Monoclonal | Mouse | Sc390381 | E2720 | 1:1000 |
SDHB | SCBT | Monoclonal | Mouse | Sc-271548 | L2619 | 1:1000 |
SDHC | SCBT | Monoclonal | Mouse | SC-515102 | F0719 | 1:1000 |
VDAC1(B-6) | SCBT | Monoclonal | Mouse | Sc-390996 | D1918 | 1:1000 |
Gene | Forward | Reverse | Amplicon (base pair) |
|---|---|---|---|
Multiplex/qRT-PCR primers | |||
SDHA | CAGTCAAGGCGAAAGGTTTATG | CCCAGCGTTTGGTTTAATTGG | 508 |
SDHB | TCCGAAGATCATGCAGAGAAG | TACAGCAGGCACAGAGAATG | 312 |
SDHC | CCCAGCATCATCTTCCTACAC | TGCAGCCACCTCATCTTTAG | 202 |
SDHC* | GTGGCACTGGTATTGCTTTG | CACAGAGCTGGCATTGTTTC | 488 |
SDHD | AGCTCTGTTGCTTCGAACTC | CAGATGCCCACATCGTGATAG | 399 |
Dicer-substrate short interfering RNAs (DsiRNAs): sense and antisense | ||
|---|---|---|
SDHB | GUAUUGGAUGCUUUAAUCAAGAUTA | UAAUCUUGAUUAAAGCAUCCAAUACCA |
Control | CCUUCCUCUCUUUCUCUCCCUUGUG | CACAAGGGAGAGAAAGAGAGGAAGG |