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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: CAMKK2 regulates mitochondrial function by controlling succinate dehydrogenase expression, post-translational modification, megacomplex assembly, and activity in a cell-type-specific manner

Fig. 7

Loss of CAMKK2 differentially affected PTMs of OXPHOS, specifically SDHA in a cell-type-specific manner. A–D Immunoblots showing charged fractions of OXPHOS proteins. H–K The immunoblots presented in A–D were treated with a rabbit monoclonal anti-SDHA antibody. Therefore, the previously detected proteins using mouse monoclonal anti-OXPHOS antibodies may show some variation compared to A-D due to loss of mouse antibodies after prolonged incubation and washing. E, L The immunoblots presented in A-D (only SDHB fractions) and H–L were false colored and overlapped. “+”: pH/pI-10. F Plot profiles of the SDHB charged fractions. Fraction 1and 2 indicates same protein with different sets of modifications. G Bar graphs showing relative percentage of fraction 1 and 2 of SDHB protein. Statistical significance from one-way ANOVA followed by multiple comparisons. M–N Immunoblots showing charged fractions of SDHC. N False colored overlay of immunoblots presented in M. Interpretation of colored rectangles and arrows: Difference in charged fractions of MT-CO2 (green dotted rectangles), NDUFB8 (red dotted rectangles) and SDHC (black/white dotted rectangles)

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Cell Communication and Signaling

ISSN: 1478-811X