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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: CAMKK2 regulates mitochondrial function by controlling succinate dehydrogenase expression, post-translational modification, megacomplex assembly, and activity in a cell-type-specific manner

Fig. 3

Fractionation of enriched ER/mitochondria. A–C: Immunoblots showing presence of CAMKK2, VDAC1, ATP5A and UQCRC2 proteins in subcellular fractions derived from HEK293 and HepG2 cells. Individual lanes under each category represent multiple replicates from a single set of experiments. WT: wild-type (parental), KO: CAMKK2 knockout, M: molecular weight ladder, and ns: nonspecific band. Yellow arrow indicates that the blot was incubated with a different set of antibodies without stripping. D–L Immunoblots showing abundance of cytosolic GAPDH and α-tubulin, mitochondrial ATP5A, SDHB, UQCRC2 and VDAC1, and ER-associated CALR [108] proteins in the subcellular fractions derived from parental (WT) and CAMKK2−/− (KO) HEK293 (D–G) and HepG2 (H–L) cells, respectively. “ × ”: Blank lane. The yellow arrows indicate longer exposure of the corresponding immunoblots. The cyan arrow indicates incubation of the corresponding blot with anti-VDAC1 antibody after striping anti-GAPDH. The red and blue rectangles indicate relatively increased or decreased SDHB levels

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Cell Communication and Signaling

ISSN: 1478-811X