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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: R-Ras subfamily proteins elicit distinct physiologic effects and phosphoproteome alterations in neurofibromin-null MPNST cells

Fig. 5

DN R-Ras inhibits phosphorylation of ROCK1 and MYPT1 and activation of RhoA while ROCK inhibition, like DN R-Ras expression, impedes migration. a Levels of total and phosphorylated ROCK1 and MYPT1 in ST88-14 and STS-26T cells stably transfected with doxycycline-inducible plasmids expressing DN R-Ras in the presence (+) and absence (−) of DN R-Ras expression. Blots were probed with an anti-actinin antibody as a loading control. Quantification indicated below the blots represents the ratio of the normalized phosphorylated to total protein levels. b Rhotekin pull-down assays in ST88-14 and STS-26T cells stably transfected with doxycycline-inducible plasmids expressing DN R-Ras. Induction of DN R-Ras expression (+) decreases RhoA activation. c Induction of DN R-Ras expression and treatment with the ROCK1 inhibitor Y27632 similarly reduce the migration of ST88-14, STS-26T, and T265 cells in Transwell migration assays. A combination of DN R-Ras expression and Y27632 treatment produces an additional small, but statistically significant reduction in migration. **, p-value < .0001; *, p-value < .05

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Cell Communication and Signaling

ISSN: 1478-811X