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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: R-Ras subfamily proteins elicit distinct physiologic effects and phosphoproteome alterations in neurofibromin-null MPNST cells

Fig. 2

Assessment of the regulation of doxycycline-inducible plasmids expressing eGFP or DN R-Ras stably tranfected into MPNST cells. a, b Expression of eGFP (a) or HA-tagged DN R-Ras (b) in stably transfected ST88-14, STS-26T and T265-2c cells 72 h after the cells were challenged with 2 μg/ml doxycycline. c Raf-1 RBD pulldown assays in ST88-14 MPNST cells stably transfected with doxycycline-inducible vectors expressing DN R-Ras or DN H-Ras and transiently transfected with a plasmid expressing Myc-tagged H-Ras. DN H-Ras, but not DN R-Ras, inhibits H-Ras activation

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Cell Communication and Signaling

ISSN: 1478-811X