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Table 1 Sequence of primers used in qPCR

From: Inhibition of protein tyrosine phosphatase improves mitochondrial bioenergetics and dynamics, reduces oxidative stress, and enhances adipogenic differentiation potential in metabolically impaired progenitor stem cells

Gene

Primer

Sequence 5′–3′

Amplicon length (bp)

Accession No

PPARγ

F:

R:

TCCCTGTTTGTGTACAGCCTT

CTCCATGGCTGATTTCCCCT

191

XM_014846252.1

ADIPOQ

F:

R:

GGAGATCCAGGTCTTGTTGG

TCGGGTCTCCAATCCTACAC

162

XM_014843352.1

Lep

F:

R:

CACACGCAGTCAGTCTCCTC CGGAGGTTCTCCAGGTCAT

176

XM_014854289.1

SREBP-1c

F:

R:

TCAGCGAGGCGGCTTTGGAGCAG

CATGTCTTCGATGTCGGTCAG

80

XM_008542859.1

SOD1 (Cu/Zn SOD)

F:

R:

CATTCCATCATTGGCCGCAC

GAGCGATCCCAATCACACCA

130

NW_001867397.1

SOD2 (Mn SOD)

F:

R:

GGACAAACCTGAGCCCCAAT

TTGGACACCAGCCGATACAG

125

NW_001867408.1

CAT

F:

R:

ACCAAGGTTTGGCCTCACAA

TTGGGTCAAAGGCCAACTGT

112

XM_014851065.1

PPARGC1β

F:

R:

CCTCAACTATCTTGCCGACACC

ATGGGTTCAGTCTCGGGGT

165

XM_023617445.1

OXA1L

F:

R:

GACCTAGAAACCGTGGGACG

GGAAGATCACTTGGCTCCCC

105

XM_008528958.1

MRPL24

F:

R:

ATGATCCCTAGCGAAGCACC

TGTAGAGACTCGTACCCGCT

123

XM_001500466.4

MTERF4

F:

R:

CGCCACCTCCGTGCTATG

CCCAAATGAGGGGCATCAGG

147

XM_023644068.1

PUSL1

F:

R:

TCAGCCACTTCCAGGACCTA

AGCCACATCCAAGCTGTCTG

120

XM_023636046.1

TFAM

F:

R:

ATGATGGCTTTGAGTCCAGG

CTAGATGATGGCGGGAGACTT

154

XM_023643450.1

NDUFA9

F:

R:

TTGGTATTCAGGCCACACCC

GCTGGCTTCACGTCTTCAAC

103

XM_001494601.4

UQCRC2

F:

R:

TGCTTCGTCTTGCATCCAGT

AACTCCGGTGACGTGGTAAC

193

XM_001494381.5

COX4I1

F:

R:

GAATAGGGGCACGAACGAGT

GCCACCCACTCCTCTTCAAA

138

XM_023637444.1

COX4I2

F:

R:

CCCCACCCCCAGATGTTCT

CGTGGTAGTTGGTGTAGGGG

136

XM_005604417.3

PIGBOS1

F:

R:

GTTGGGGTGGCTCAGATCAA

ACCCCTCCTTTACCGCTACT

126

XM_014733689.2

MIEF1

F:

R:

GTGAGCGCAAAGGCAAGAAA

CTTAACCGCCAGTGTAGCGA

128

XM_023631522.1

PINK1

F:

R:

GCACAATGAGCCAGGAGCTA GGGGTATTCACGCGAAGGTA

289

XM 014737247.1

PARKIN

F:

R:

TCCCAGTGGAGGTCGATTCT CCCTCCAGGTGTGTTCGTTT

218

XM 014858374.1

MFN1

F:

R:

AAGTGGCATTTTTCGGCAGG TCCATATGAAGGGCATGGGC

217

XM 001495170.5

MFN2

F:

R:

AGGTGAAGTCAGAATTGGTGGA CTTCACAGGGGTGGCATCAT

129

XM_023635773.1

Fis

F:

R:

GGTGCGAAGCAAGTACAACG GTTGCCCACAGCCAGATAGA

118

XM 001504462.4

MIRO1

F:

R:

GATCCTGCTGGTGGGAGAAC

GGGAGGAACCTCTTCTGGGA

88

XM_023651639.1

OPA1

F:

R:

CTTCTCTTGTTAGGTTCACCTGG

TGTAAGAGAATGAGCTCACCAAG

110

XM_003363363.4

DRP1

F:

R:

CAGCTAGTCCACGTTTCACC CCCTTTAGAAAGGTGTCTTGAGT

96

XM_023643346.1

LC3

F:

R:

TTCTGAGACACAGTCGGAGC

CTTTGTTCGAAGGTGTGGCG

128

XM_001493613.6

Beclin

F:

R:

GATGCGTTATGCCCAGATGC

AACGGCAGCTCCTCTGAAAT

233

XM_014833759.1

Lamp2

F:

R:

GCACCCCTGGGAAGTTCTTA

ATCCAGCGAACACTCTTGGG

147

XM_014729146.2

GAPDH

F:

R:

GATGCCCCAATGTTTGTGA

AAGCAGGGATGATGTTCTGG

250

NM_001163856.1

  1. Sequence: amplicon length and access numbers of the primer sets. PPARγ: Peroxisome proliferator-activated receptor gamma; ADIPOQ: Adiponectin; Lep: Leptin; CEBPA: CCAAT/enhancer-binding protein alpha; SREBP-1C: Sterol Regulatory Element Binding Protein-1c; AKT1: Serine-threonine protein kinase 1; AKT2: Serine-threonine protein kinase 2; INSR: Insulin receptor; IRS1: Insulin Receptor substrat 1; PI3K: Phosphoinositide 3-Kinase; SHBG: Sex hormone binding globulin; AHSG: Alpha 2-HS Glycoprotein; P53: tumor suppressor p53; Bcl-2: B-cell lymphoma 2; Bax: BCl-2 associated X protein; p21: Cyclin-dependent kinase inhibitor 1; Casp3: Caspase 3; Casp9: Caspase 9; Perk: PRKR-like endoplasmic reticulum kinase; Chop: C/EBP homologous protein; Atf6: Activating transcription factor 6; Ire1: Inositol-requiring enzyme; Xbp1: X-box binding protein 1Sod1 (Cu/Zn SOD): Copper-zinc-dependant superoxide dismutase (CuZnSOD; Sod2 (Mn SOD): Manganese-dependent superoxide dismutase (MnSOD); CAT: Catalase; PPARGC1β: Peroxisome proliferator-activated receptor γ coactivator 1-beta; OXA1L: OXA1L, mitochondrial inner membrane protein; MRPL24: Mitochondrial Ribosomal Protein L24; MTERF4: Mitochondrial Transcription Termination Factor 4; PUSL1: Pseudouridine Synthase Like 1; TFAM: Mitochondrial transcription factor A; NDUFA9: NADH ubiquinone oxidoreductase subunit A9; UQCRC2: Ubiquinol Cytochrome C Reductase Core Protein 2; COX4I1: Cytochrome c oxidase subunit 4 isoform 1; COX4I2: Cytochrome c oxidase subunit 4 isoform 2; PIGBOS1: PIGB, opposite strand protein 1; MIEF1: Mitochondrial Elongation Factor 1; PINK1: PTEN-induced kinase 1; PARKIN: Parkin RBR E3 ubiquitin protein ligase (PARK2); MFN1: Mitofusin 1; MFN2: Mitofusin 2; FIS1: mitochondrial fission 1 molecule; MIRO1: Ras Homolog Family Member T1; OPA1: OPA1, Mitochondrial Dynamin Like GTPase; DRP1: Dynamin-1-like protein; GADPH: glyceraldehyde-3-phosphate dehydrogenase
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Cell Communication and Signaling

ISSN: 1478-811X