Skip to main content
Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Inhibition of protein tyrosine phosphatase improves mitochondrial bioenergetics and dynamics, reduces oxidative stress, and enhances adipogenic differentiation potential in metabolically impaired progenitor stem cells

Fig. 7Fig. 7

Analysis of mitochondrial morphology, mitophagy, and autophagy during adipogenic differentiation. Images showing mitochondrial networks by cells analysed by MicroP software (a). Based on the MicroP data, mitochondrial morphology in cells was established (b). Additionally, the data was displayed as a heat map (c). Representative bands (d) show the results of western blot. The amount of MFF (e), PINK-1 (f), and MFN-1 (g) was established. Using RT-qPCR, expression of genes involved in mitophagy and autophagy-PINK-1 (h), PARKIN (i), BECLIN-1 (j), LC3 (k) and LAMP-2 (l) was investigated. Results are expressed as mean ± SD; n = 3. p < 0.05, p < 0.01, and p < 0.001. Statistical significance indicated as asterisk (*) when comparing the result to ASCs HE and as number sign (#) when compared to ASCs EMS

Back to article page