Fig. 3

SHP2 increases robustness of basal and early IL-6-induced STAT3 phosphorylation. a, b Single cell STAT3 expression and STAT3-Y705 phosphorylation in unstimulated MEF wt cells (A) and MEF wt cells stimulated with 150 ng Hy-IL-6 per ml for 15 min (B). c Robustness negatively correlates with Mutual Information. d Based on the data presented in Fig. 2a–f Mutual Information between STAT3 expression and cytokine-independent STAT3-Y705 phosphorylation was calculated in unstimulated MEF wt (blue), MEF SHP2 ΔEx3 (green), and MEF SHP2 ΔEx3 + SHP2 cells (violet). Statistics: Stars indicate significant differences between the cell lines. p-value < 0.05 (Anova) and d > 0.2 (complete statistics for Fig. 3 in Additional file 2 Table 1) e Based on the data presented in Fig. 2a–f Mutual Information between STAT3 expression and cytokine-dependent STAT3-Y705 phosphorylation was calculated in MEF (blue), MEF SHP2 ΔEx3 (green), and MEF SHP2 ΔEx3 + SHP2 cells (violet) stimulated with the indicated amounts of Hy-IL-6 for 15 min. Data are from n = 3 independent experiments. Statistics: Stars indicate a significant increase of MI in Hy-IL-6-treated cells compared to MI in unstimulated cells (Fig. 3d) within each cell line: blue * (wt), green * (ΔEx3), violet * (Δ Ex3 + SHP2) p-value < 0.05 (Anova) and d > 0.2 (complete statistics for Fig. 3 in Additional file 2: Table 1)