Fig. 3

MB49-derived exosomes play a major role in inducing the immunosuppressive function of macrophages. BMDM were treated with MB49-derived exosomes, which were extracted from MB49 CM ± GW4869, for 12 h or 24 h respectively. A The expression of Il10, Cd206, Tgfb and Inos qRT-PCR was measured by qRT-PCR. B The expression of CD63 and Hsp90 was analyzed by Western blot. C The expression of Il10, Cd206 and Inos was quantified by qRT-PCR. D BMDM were stimulated with exosomes for 48 h and flow cytometry were used to detect the percentage of F4/80⁺CD206⁺ macrophages, the representative plot was shown. E The exosome stimulated BMDM were further co-cultured with CFSE labeled splenic T cells for 3 d, the percentage of CFSE⁺ CD4⁺ or CD8⁺ T cells was measured by flow cytometry. The experiments were repeated for 3 times. Data are present as mean ± SD, one-way ANOVA, *P < 0.05; **P < 0.01; ***P < 0.001