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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Cancer derived exosomes induce macrophages immunosuppressive polarization to promote bladder cancer progression

Fig. 2

Identification of MB49-derived exosomes and their uptake in macrophages. A The morphology of exosomes isolated from MB49 cells were observed by electron microscope. Shown in the box are typical exosome particles. Scale bar, 500 nm. B The expression of CD63 and HSP90 were detected by Western blot analysis in MB49 exosomes. C Cy5.5-labeled exosomes were co-cultured with BMDM for 3 h, and the uptake of exosomes was observed under a confocal microscope. The representative images were shown. Scale bar, 10 μm. D The mean fluorescence intensity (MFI) of Cy5.5-labeled exosomes treated BMDM was measured by flow cytometry, and the control group was treated with PBS-exosomes. E Cy5.5-labeled MB49-derived exosomes or PBS MB49-exosomes were injected into mice via the tail vein. And the MFI of Cy5.5 labeled exosomes in CD45+F4/80+CD11b+ bladder macrophages was analyzed by flow cytometry. Each experiment was repeated three times, and the data were expressed as mean ± SD, t-test, **P < 0.01

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