Fig. 3
From: Pharmacologic targeting of the P-TEFb complex as a therapeutic strategy for chronic myeloid leukemia
Wogonin induced apoptosis in KU-812 cells through downregulating MCL1. a KU-812, K562 and #1 primary CML cells were treated with Wogonin (0, 5, 10, 20, 40 and 80 μM) for 48 h. Flow cytometric analysis of Annexin V/PI stained cells. Data represent the mean ± SD of three independent experiments. b Quantification of apoptosis cells. Columns represent the mean from three parallel experiments (mean ± SD). *p < 0.05, **p < 0.01, compared with control group. c The mRNA level of MCL1 in KU-812, K562 cells and #1 primary CML cells after cells were treated with wogonin (0 and 80 μM) for 48 h. Columns represent the mean from three parallel experiments (mean ± SD). **p < 0.01, compared with control group. d The protein expression level of MCL-1 in KU-812, K562 cells and #1 primary CML cells after cells were treated with various concentrations of wogonin for 48 h. β-actin and GAPDH was used as a loading control respectively. The results are representative of three independent experiments. e Quantification of the protein expression level of MCL-1 in KU-812, K562 cells and #1 primary CML cells. Columns represent the mean from three parallel experiments (mean ± SD). *p < 0.05, **p < 0.01, compared with control group