Fig. 1

Hhex was downregulated and inhibited cell migration in lung cancer cells. a Box plots of Hhex mRNA levels determined from four Oncomine datasets, namely Hou Lung, Landi Lung, Okayama Lung and Selamat Lung, (***P < 0.001; P values were obtained using two-tailed Student’s t-tests). b KM plotter analysis of the relation between Hhex gene expression and (OS) in lung cancer patients. c Control siRNA (CTRL) or HHEX siRNA (#1) and (#2) was transfected into H1792 cells. When cells reached monolayer confluency, all cells were treated with proliferation inhibitors mitomycin-C (10 μg/ml)1 h prior to performing the scratch assay. And the images of these scratched sites were obtained every 12 h to recorded width changes. The picture showed above represented time points at 0 h and 24 h for each group. Western blotting of 24 h whole-cell extracts was performed. And t-test was used to analyze the differences between the treatment groups. Data are presented as means ± S.D. *P < 0.05; **P < 0.01 (n = 5) (d) A549 cells were treated similar to H1792 cells. The picture showed above represented time points at 0 h and 48 h for each group. Data are presented as means ± S.D. *P < 0.05; **P < 0.01 (n = 5) (e), f Hhex was overexpressed in H1792 cells and A549 cells, then wound-healing scratch experiments were performed as same as (c, d), data are presented as means ± S.D. *P < 0.05; **P < 0.01 (n = 5)