Fig. 6

Western blot analysis of major vault protein (MVP) and annexin A2 (ANXA2) in primary cortical neurons and astrocytes. a Representative images of the protein expression of MVP, ANXA2, and loading control β-actin in neurons and astrocytes. b Quantification of MVP and ANXA2 expression (normalized to the expression of β-actin) is shown as mean ± standard deviation. n = 3 independent experiments. ***p-value < 0.0005; ns: not significant; two-tailed unpaired t-test. Twenty μg of total protein was loaded per lane. c Representative images showing the expression of MVP and ANXA2 in primary cortical neurons, astrocytes, and their sEVs (n = 2). Ten μg of total protein was loaded per lane. NC: neurons, AC: astrocytes, NE: sEVs from neurons, AE: sEVs from astrocytes