Fig. 2
From: Differential RNA packaging into small extracellular vesicles by neurons and astrocytes
Characterization of sEVs derived from primary neurons and astrocytes. a Nanoparticle tracking analysis (NTA) of neuronal sEVs showing a mean diameter of 137.2 ± 2.7 nm and a concentration of 1.07 × 1010 ± 8.51 × 108 particles/mL. b NTA showing astrocytic sEVs with an average diameter of 135.6 ± 2.2 nm and concentration of 5.68 × 109 ± 2.71 × 108 particles/mL. c Transmission electron microscopy (TEM) confirmed that most sEV diameters were to be less than 150 nm and immunogold labeling showed the presence of CD81. Scale bars = 100 nm. d Western blot analysis of three independent experiments showed that sEVs were enriched in EV markers HSP70, CD63 and CD81. Calnexin, an endoplasmic reticulum/Golgi marker, was present only in cell lyases but absent in sEV preparations, confirming the purity of sEVs. Ten μg of total protein was loaded per lane. NC: neurons, AC: astrocytes, NE: sEVs from neurons, AE: sEVs from astrocytes