Fig. 1

Purity of neuronal and astrocytic cultures. a The purity of the neuronal culture was confirmed using neuronal marker MAP2 (microtubule associated protein 2) (red) and absence of astrocytic marker GFAP (glial fibrillary acidic protein) (green). Most cells with nuclei marker DAPI (blue) were also stained with MAP2, while few green GFAP labeled astrocytes were observed. b The astrocytic culture was enriched in GFAP (green) while the microglial marker CD-11b (red) was almost completely absent, indicating purity of DAPI-stained astrocytes. Scale bars = 100 μm. c Quantification of percentage purity of neuronal cultures (MAP2A⁺ cells, left) and astrocytic cultures (GFAP⁺ cells, right) in the total number of cells stained with DAPI. The data represents three independent experiments and are presented as mean ± standard deviation