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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: PML-II regulates ERK and AKT signal activation and IFNα-induced cell death

Fig. 5

PML-II depletion increases AKT signaling. A HeLa cells were transfected and stimulated as Fig. 4C, and after the indicated times with IFNα, cells were lysed and the expression of p-AKT and p-p70S6K1 was detected by western blotting. B HeLa cells were stimulated with 1000 U/ml or 5000 U/ml IFNα for 72 h, then cells were lysed for WB testing appropriate proteins. C The graph shows the quantification of WB bands in (B) by using the Odyssey system (Pierce), normalized to β-actin and expressed relative to non-IFNα stimulation. D Cells were treated as Fig. 4C, and were collected at different time points for testing p-ATK by western blotting. E The bands of p-AKT in (D) were quantified by using the Odyssey system, values normalized to β-actin and expressed relative to control siRNA cells treated without IFNα stimulation

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