Fig. 4
From: PML-II regulates ERK and AKT signal activation and IFNα-induced cell death
PML-II regulates ERK signaling. (A, B) HeLa cells were transfected with siPML-II or control siRNA, and after 48 h cells were collected. A Total protein lysates were analyzed by western blotting for p-ERK, p-AKT and p-p70S6K1. B Total cell RNA was analyzed by SYBR Green qPCR for c-Fos and c-Myc mRNA expression; amounts are expressed relative to GAPDH and normalized to the siControl sample. Error bars show standard deviation among technical replicates. C, D HeLa cells were transfected with siPML or control siRNA for 48 h, then stimulated with 1000 U/ml IFNα and collected at the described time points. Total protein lysates were analyzed by western blotting for ERK, p-ERK and p-AKT as indicated. Panel C is taken from the same experiment as Fig. 1C; the control β-actin data are duplicated for ease of reference