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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: PML-II regulates ERK and AKT signal activation and IFNα-induced cell death

Fig. 3

PML-II depletion downregulates pro-apoptotic gene expression. Hela cells were transfected with either PML-II siRNA or control siRNA for 48 h. Cells were stimulated with 1000 U/ml IFNα and were collected at described time points. (AC, E, G, H) The mRNA expression levels of A PML-II, B ISG54, C TRAIL, E PUMA, G Bcl2 and H Bcl-xL were measured by SYBR Green qPCR. (D, F) The expression levels of D TRAIL and F PUMA proteins were detected by western blotting (left panels) and quantified by Odyssey system relative to β-actin (right panels). Error bars show standard deviation among technical replicates

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