Fig. 2
From: PML-II regulates ERK and AKT signal activation and IFNα-induced cell death
IFNα induces pro-apoptotic gene expression. HeLa cells were stimulated with 1000 U/ml IFNα and collected at different time points. (A–F) The mRNA levels of A ISG15, B ISG54, C OAS1, D TRAIL, E PUMA, F MCL-1 and G BCL-2 were measured by SYBR Green qPCR. The mRNA level of each gene was quantified relative to GADPH and normalized to the time zero value. Results are presented as mean ± SD of technical triplicate experiments. H–I Whole cell protein was analyzed by western blotting for ISG15, ISG54, OAS1, PML, p-STAT1, TRAIL, BCL-2 and MCL-1. β-actin was used as loading control. (J–K) The protein bands in panel H and I were visualized and quantified with an Odyssey system (Pierce), normalized to β-actin