Fig. 7

Cell–cell ligand–receptor network analysis. a Comparison of total incoming path weights vs total outgoing path weights across BAI cell populations. b Circle network diagram of significant cell–cell interaction pathways. Arrows and edge color indicate direction (ligand: receptor) and edge thickness indicates the sum of weighted paths between populations. c–e Heatmap showing the interaction weights calculated as the product of the average ligand expression from the source cell type including monocyte/ DC (c), epithelial cell (d) macrophages (e), to the average receptor expression of the target cell types. Grey boxes indicate interactions that are not significantly present across all cell types (one-sided Wilcoxon rank-sum test and Benjamini Hochberg false discovery rate [FDR] > 0.05). f Heatmap showing the global five communication patterns calculated by the key signals for subpopulations between macrophage and T cells. g Hierarchical network diagram of significant cell-cell communication patterns. Edge thickness indicates the sum of weight key signals between populations (from outgoing to incoming)