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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Effect of HSP90AB1 and CC domain interaction on Bcr-Abl protein cytoplasm localization and function in chronic myeloid leukemia cells

Fig. 2

Association between the Bcr/Abl CC-domain and N-terminal domain of HSP90AB1. a DYK-tagged HSP90AB1 and/or HA-tagged Bcr-Abl were overexpressed in 293 T cells and subjected to reciprocal coimmunoprecipitation (Co-IP) to detect protein interaction between Bcr-Abl and HSP90AB1. *p < 0.05 and ***p < 0.001. b The three-dimensional structure diagram of Bcr/Abl-HSP90AB1 binding protein was simulated using the bioinformatics analysis software Deep Viewer. c The target gene expression vectors DYK-pAdTrack-HSP90AB1 and DYK-pAdTrack-HSP90AB1-ΔNTD were successfully constructed by using molecular cloning technology. The target genes HSP90AB1 and HSP90AB1-ΔNTD were amplified by PCR technology, and their bases were 2176 bp and 1483 bp. d Immunofluorescence was used to observe the transfection efficiency of the DYK-pAdTrack-HSP90AB1 vector with the fluorescent label in 293 T cells at 6 h, 24 h, and 48 h. e, f 293 T cells expressing HA-tagged Bcr-Abl, DYK tagged HSP90AB1 or DYK-HSP90AB1-ΔN were subjected to coimmunoprecipitation using anti-Flag beads

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