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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: PIM kinases inhibit AMPK activation and promote tumorigenicity by phosphorylating LKB1

Fig. 2

PIM expression levels inversely correlate with AMPK phosphorylation levels. PIM family members were knocked out in PC3 and MCF7 cells. a PIM expression levels in wild-type (WT) cells or their knock-out derivatives lacking individual (PIM1, PIM2, PIM3 KO) or all three (TKO) PIM kinases were examined by Western blotting. ACTB staining was used as a loading control. NS refers to non-specific staining observed with the PIM1 antibody. Phospho-AMPK (Thr172) versus AMPK levels were measured from WT cells in comparison to cells lacking individual b or all c PIM family members (average values ± SD, n = 3). d WT or TKO cells were transiently transfected with His or His-PIM1 plasmids, lysed 24 h later and subjected to Western blotting to determine relative AMPK phosphorylation levels (average values ± SD, n = 3). e Lysates of stably transfected FD/NEO and FD/PIM1 cell lines were subjected to Western blotting to determine relative AMPK phosphorylation levels

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