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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: The phosphatase Shp1 interacts with and dephosphorylates cortactin to inhibit invadopodia function

Fig. 4

Shp1-induced inhibition of ECM degradation in the presence of the indicated glycerophosphoinositols in A375MM cells. A ECM degradation levels evaluated in non-targeting siRNAs and Shp-1 siRNAs-treated A375MM cells plated on gelatin-coated coverslips and incubated in the absence and presence of 50 μM GroPIns and GroPIns4P for 16 h. Representative pictures are shown in Additional file 1: Figure S2. B ECM degradation levels evaluated in control, Shp1 WT, and Shp1C455S-transfected cells plated on gelatin-coated coverslips and incubated in the absence and presence of 50 μM GroPIns and GroPIns4P for 16 h. Representative pictures are shown in Additional file 1: Figure S3. Data are expressed as the means (± SE) of at least three independent experiments performed in duplicate and indicate the total area of degradation (expressed as percentages of control). ***P < 0.001; **P < 0.02; *P < 0.05; ns P > 0.05 (Student’s t-test) calculated for each treatment versus the respective untreated control, as indicated. Note that the P-value was also calculated for the other samples to compare the different effects of each treatment according to the presence/absence of Shp1 in the system: * for Non-targeting Ctr versus Shp1 siRNAs Ctr; *** for Non-targeting GroPIns versus Shp1 siRNAs GroPIns; ns for Non-targeting GroPIns4P vs Shp1 siRNAs GroPIns4P. C Representative pull-down of streptavidin-conjugated beads using Biotin or biotinylated GroPIns (GroPIns-Bio) with either Shp1 1–529 (His-Shp1), the N-terminal SH2-domain mutant (His-SH2 (N + C)) or the catalytic-domain mutant (His-PTPase) of Shp1. Eluted (beads) proteins were analyzed by western blotting using an anti-Histidine antibody. Molecular weights (kDa) are indicated on the left of each panel. D Schematic domain structure illustrating the amino acid sequences of the Shp1 mutants used in the pull-down

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