Fig. 1

Altering composition of the ARF19 IDR impacts the morphology of ARF19 condensates. A Schematic of the ARF19 protein showing the location of the DBD and PB1 domains. The graph below shows predicted prion-like domains within the ARF19 IDR, which were predicted using PLAAC [61]. B Schematic showing a subsection of the ARF19 IDR highlighting differences in IDR composition for the QtoS (middle) or QtoG (bottom) variants. While this schematic only shows a subsection of the IDR, for the QtoG or QtoS variants, all glutamines were changed to glycine or serine, respectively. C Images showing the range of condensate morphologies formed by wild-type ARF19 or ARF19 with the altered IDR compositions. Images were chosen to represent the breadth of condensate morphology and sizes that are frequently observed across protoplasts when expressing the various constructs. All images were taken in different protoplasts approximately 16 h after transfection. Scale bars represent 5 microns