Fig. 4
From: Mitochondrial DNA leakage induces odontoblast inflammation via the cGAS-STING pathway
LPS induced mitochondrial injury and mtDNA leakage into the cytosol in mDPC6T cells. a Western blotting was performed to assess the expression levels of TOMM20, VDAC1 and TFAM in mDPC6T cells after stimulation with LPS (20 μg/ml, 24 h). b Mitochondrial superoxide was evaluated by MitoSOX Red staining in mDPC6T cells after stimulation with LPS (20 μg/ml, 24 h). The box plot shows the signal intensity (n = 20). c Immunofluorescent double-labeling of DNA and mitochondria in mDPC6T cells after stimulation with LPS (20 μg/ml) for 24 h was observed by confocal microscopy. Mitochondria was stained with MitoTracker (red). Double-stranded DNA was stained with anti-dsDNA antibodies (green). Scale bar: 10 μm. d mtDNA copy number in the cytoplasm was assessed by real-time PCR in mDPC6T cells after LPS stimulation (20 μg/ml, 24 h). The data are the mean ± SEM, n = 3 (*p < 0.05, **p < 0.01 ***p < 0.001)