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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: The extracellular HDAC6 ZnF UBP domain modulates the actin network and post-translational modifications of Tau

Fig. 6

Modulation of ApoE and Tubulin localization by HDAC6. a Immunofluorescence mapping for ApoE and Tau upon HDAC6 treatment in neuro2a cells shows increased nuclear localization of ApoE. b ApoE intensity in nucleus and cytoplasm was quantified for untreated and HDAC6 ZnF UBP treated neuro2a immunostained with anti-ApoE antibody. There was no significant difference in cytoplasmic level of ApoE in both groups while nuclear ApoE fraction was notably increased in HDAC6 ZnF UBP treated cells. c Neuro2a treated with HDAC6 ZnF UBP shows localization of tubulin focused over nuclear periphery compared to untreated neuro2a where tubulin is evenly distributed in cytoplasm. Orthogonal projection image shows tubulin localization in nuclear periphery in HDAC6 ZnF UBP treated neuro2a cells. Statistical significance determined by two-tailed unpaired t-test. (n.s.—non-significant, * indicates P ≤ 0.05, ** indicates P ≤ 0.01, *** indicates P ≤ 0.001)

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Cell Communication and Signaling

ISSN: 1478-811X