Fig. 3

HDAC6 as a modulator of actin dynamics. HDAC6 deacetylase activity is known to modulate actin dynamics. The effect on actin dynamics was studied after exposure to HDAC6 ZnF UBP domain. HDAC6 ZnF UBP treatment to neuro2a resulted in enhancement of neurite extensions which are mapped by F-actin staining. a HDAC6 ZnF UBP treatment for 24 h increases the neuronal extensions as compared to untreated control cells when the extensions are mapped by actin immunostaining. The Tau levels remain unaltered in both the groups. b The orthogonal projection shows increase in the number of neurites in HDAC6 ZnF UBP treated cells. c Greyscale images for untreated neuro2a cells mapped for F-actin showed small extensions in the growth cones. d In HDAC6 ZnF UBP treated cells, F-actin containing longer extensions were observed. Growth cones were observed to be concentrated in membrane extensions and filopodia-like structures