Fig. 2

Inhibition of Tau phosphorylation by HDAC6. a HDAC6 treatment for 24 h along with OA shows inhibition of Tau phosphorylation at pT181 epitope whereas OA treatment shows increased phosphorylation at pT181. b Phosphorylation at AT8 epitope is also reduced in presence of HDAC and OA as compared to OA alone. The enlarged image of neuro2a treated with OA alone and OA along with HDAC6 ZnF UBP shows marked difference in the level of phosphorylation at pT181 and AT8 epitopes. c, d Mean fluorescence intensity for AT8 immunostaining of untreated neuro2a (CC), okadaic acid treatment (OA), HDAC6 ZnF UBP treatment (HDAC6) and HDAC6 ZnF UBP treatment along with okadaic acid. Okadaic acid treatment resulted in increased level of pT181 and AT8 phospho-epitopes. HDAC6 ZnF UBP treatment with OA reduced the level of both phospho-epitopes. Quantification data was analyzed by one way ANOVA followed by Tukey’s HSD test. Significant at mean difference > T (Tukey’s criterion) and α = 0.05. Groups with the same letters are not significantly different. The value of T is calculated as 0.365 for pT181 and 1.341 for AT8 quantification