Skip to main content

Table 2 List of antibodies employed for proteins profiling using western blot analysis

From: Hyoscyamus albus nortropane alkaloids reduce hyperglycemia and hyperinsulinemia induced in HepG2 cells through the regulation of SIRT1/NF-kB/JNK pathway

Antibody Dilution Catalog No
Insr 1:1000 Invitrogen, MA1-10865
Akt Pan 1:1000 Invitrogen, 44-609G
Pi3kCD 1:1000 Invitrogen, PA5-83748
SIRT1- N-terminal region 1:1000 ARP32386
TOR/mTOR 1:500 nb100-240
NF-kappaB p65(phospho-T254) 1:1000 Biorbyt, orb304547
JNK1/JNK2(phospho-Thr183/Tyr185) 1:1000 Biorbyt, orb15028
Actin 1:2000 Sigma Aldrich, a2066
  1. Insr, Insulin Receptor; Akt, Protein Kinase B; Pi3kCD, Phosphatidylinositol 3-Kinase; SIRT1, Sirtuin 1; TOR/wTOR, Target of rapamycin/Mammalian Target of rapamycin; NF-kappaB p65(phospho-T254), Phosphorylated Nuclear Factor-kappa-B transcription factor p65; JNK1/JNK2(phospho-Thr183/Tyr185), Phosphorylated c-Jun N-terminal kinase
Back to article page

Cell Communication and Signaling

ISSN: 1478-811X