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Table 1 Sequences of primers used in qPCR

From: Hyoscyamus albus nortropane alkaloids reduce hyperglycemia and hyperinsulinemia induced in HepG2 cells through the regulation of SIRT1/NF-kB/JNK pathway

Gene

Primer

Sequence 5'–3'

Amplicon length (bp)

Accession No

Mfn1

F:

R:

GTTGCCGGGTGATAGTTGGA

TGCCACCTTCATGTGTCTCC

146

NM_033540.3

Mfn2

F:

R:

AATCTGAGGCGACTGGTGAC GGACATTGCGCTTCACCTTC

126

XM_024451299.1

Parkin

F:

R:

GTGCAGAGACCGTGGAGAAA GCTGCACTGTACCCTGAGTT

294

NM_013987.3

Sod1 (cu/zn sod)

F:

R:

CATTCCATCATTGGCCGCAC

GAGCGATCCCAATCACACCA

130

NW_001867397.1

Sod2 (mn sod)

F:

R:

GGACAAACCTGAGCCCCAAT

TTGGACACCAGCCGATACAG

125

NW_001867408.1

Cat

F:

R:

ACCAAGGTTTGGCCTCACAA

TTGGGTCAAAGGCCAACTGT

112

XM_014851065.1

GPx

F:

R:

TCCGGGACTACACCCAGATG

TCTTGGCGTTCTCCTGATGC

108

NM_000581.4

TLR4

F:

R:

GACGGTGATAGCGAGCCAC

TTAGGAACCACCTCCACGCAG

173

NM_138554.5

Pink1

F:

R:

GCTTGGGACCTCTCTTGGAT

CGAAGCCATCTTGAACACAA

142

NM_032409.3

NF-kB-p65

F:

R:

CTGTTCCCCCTCATCTTCCC

GTATCTGTGCTCCTCTCGCC

113

L19067.1

c-Jun

F:

R:

ACGGCGGTAAAGACCAGAAG CCAAGTTCAACAACCGGTGC

89

NM_002228.4

IL-1β

F:

R:

AAACAGATGAAGTGCTCCTTCCAG

TGGAGAACACCACTTGTTGCTCCA

391

NM_000576.3

IL-4

F:

R:

CTTTGCTGCCTCCAAGAACAC

GCGAGTGTCCTTCTCATGGT

97

NM_000589.4

IL-6

F:

R:

TCCTTCTCCACAAACATGTAACAA

ATTTGTGGTTGGGTCAGGGG

319

NM_001318095.2

TNF-α

F:

R:

AGTGACAAGCCTGTAGCCCA GTCTGGTAGGAGACGGCGAT

242

NM_000594.4

p53

F:

R:

AGATAGCGATGGTCTGGC

TTGGGCAGTGCTCGCTTAGT

381

NM_001126118.1

Insr

F:

R:

TAGACGTCCCGTCAAATATTGC GAAGAAGCGTAAAGCGGTCC

244

AH002851.2

Irs2

F:

R:

GAGCTGTGGCGTTTCACATC AGCTTCGGGCTGAAACAGT

234

NM_003749.3

Akt1

F:

R:

CTGTCATCGAACGCACCT GTCTGGATGGCGGTTGTC

178

NM_005163.2

Akt2

F:

R:

TCAAAGAAGGCTGGCTCCAC TGTACCCAATGAAGGAGCCG

205

M95936.1

Pi3k

F:

R:

TTTAATCTGCCAGGCGGAGG CCAGAATTCCATGGGGCAGT

151

NM_006218.4

Bax

F:

R:

ACCAAGAAGCTGAGCGAGTGTC

ACAAAGATGGTCACGGTCTGCC

356

XM_011527191.1

Bcl-2

F:

R:

ATCGCCCTGTGGATGACTGAG

CAGCCAGGAGAAATCAAACAGAGG

129

NM_000633.2

p21

F:

R:

AGAAGAGGCTGGTGGCTATTT

CCCGCCATTAGCGCATCAC

169

NM_001220777.1

Casp3

F:

R:

CTCTGGTTTTCGGTGGGTGT

CTTCCATGTATGATCTTTGGTTCC

136

NM_004346.4

Casp9

F:

R:

CAGGCCCCATATGATCGAGG

CTGGCCTGTGTCCTCTAAGC

142

NM_032996.3

Sirt1

F:

R:

TCACTGCACCACTTGAGTCC ACAGGTTGCGGGAATCCAAA

73

NM_012238.5

Foxo1

F:

R:

CTCTGGCCCCTTTCACCATA GCTTTCTTCTTGGCAGCTCG

229

XM_011535010.2

G6PC

F:

R:

CACTTCCGTGCCCCTGATAA AGTATACACCTGCTGTGCCC

95

NM_000151.4

GAPDH

F:

R:

GTCAGTGGTGGACCTGACCT

CACCACCCTGTTGCTGTAGC

256

NM_001289746.1

  1. Mfn1, Mitofusin 1; Mfn2, Mitofusin 2; Parkin, Parkin RBR E3 ubiquitin protein ligase (PARK2); Pink1, PTEN-induced putative kinase 1; Sod1 (Cu/Zn SOD), Copper-zinc-dependant superoxide dismutase (CuZnSOD; Sod2 (Mn SOD), Manganese-dependent superoxide dismutase (MnSOD); CAT, Catalase; GPx, Glutathione Peroxidase; TLR4, Toll like receptor 4; NF-kB-p65, Nuclear Factor-kappa-B transcription factor p65; c-Jun, Jun Proto-Oncogene, AP-1 Transcription Factor Subunit; IL-1β, Interleukin 1 beta; IL-4, Interleukin 4; IL-6, Interleukin 6; TNF-α, Tumor Necrosis Factor alpha; P53, tumor suppressor p53; Insr, Insulin Receptor; Irs2, Insulin Receptor Substrate 2; Akt1, Serine/threonine 308 Kinase 1; Akt2, serine/threonine kinase 2; Pi3k, Phosphoinositide 3-Kinase; Bcl-2, B-cell lymphoma 2; Bax, BCl-2 associated X protein; p21, Cyclin-dependent kinase inhibitor 1; Casp3, Caspase 3; Casp9, Caspase 9; Sirt1, Sirtuin 1; Foxo1, forkhead box O1; G6PC, Glucose 6 phosphate Catalytic subunit; GADPH, Glyceraldehyde-3-phosphate dehydrogenase