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Fig. 9 | Cell Communication and Signaling

Fig. 9

From: Hyoscyamus albus nortropane alkaloids reduce hyperglycemia and hyperinsulinemia induced in HepG2 cells through the regulation of SIRT1/NF-kB/JNK pathway

Fig. 9Fig. 9

Effect of total calystegines on the NF-κB/JNK/TLR4 inflammatory pathway triggered by insulin resistance condition in HepG2 cells. a Relative gene expression of c-Jun, NF-κB and Tlr4 factors. b Quantitative representation of total NF- κB p65 and JNK1/2 proteins. c Quantitative representation of phosphorylated NF- κB p65(T254) and JNK(Thr183/Tyr185) proteins. d Representative chemiluminescent blots for analysed proteins. e Relative levels of main pro-inflammatory cytokines transcripts. Representative data from three independent experiments are shown ± SD (n = 3). An asterisk (*) indicates a comparison of IR group to untreated healthy cells. A hashtag (#) indicates a comparison of IR group pre-treated with calystegines to IR untreated healthy cells. */#p < 0.05, **/##p < 0.01, ***/###p < 0.001. HepG2_HE: HepG2 healthy untreated cells; HepG2_IR: Insulin resistant HepG2 cells exposed to high concentrations of insulin and glucose. HepG2_IR-H. albus_Caly: Insulin resistant HepG2 cells exposed to high concentrations of insulin and glucose and pre-treated with 250 μg/ml calystegines extracted from Hyoscyamus albus seeds

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