Fig. 6

regulatory potential of calystegines on Sirt/mTOR axis in the course of IR induced in HepG2 cells. a Average relative expression of Sirt1 gene normalized to houskeeping GAPDH gene. b Relative quantitative abundance of Sirt1 and mTOR proteins obtained from western blot analysis. c Representative immunoblots for each assayed protein detected by chemiluminescence. Representative data from three independent experiments are shown ± SD (n = 3). An asterisk (*) indicates a comparison of IR group to untreated healthy cells. A hashtag (#) indicates a comparison of IR group pre-treated with calystegines to IR untreated healthy cells. */#p < 0.05, **/##p < 0.01, ***/###p < 0.001. HepG2_HE: HepG2 healthy untreated cells; HepG2_IR: Insulin resistant HepG2 cells exposed to high concentrations of insulin and glucose. HepG2_IR-H. albus_Caly: Insulin resistant HepG2 cells exposed to high concentrations of insulin and glucose and pre-treated with 250 μg/ml calystegines extracted from Hyoscyamus albus seeds