Fig. 5
From: ATF5 and HIF1α cooperatively activate HIF1 signaling pathway in esophageal cancer
ATF5 acts as a novel coactivator in the HIF1 transcription complex by binding to HIF1α. a, b Examining the interaction between ATF5 and HIF1 target gene promoters by CHIP in hypoxia. c, d Investigating the interaction between HIF1 transcriptional complex and VEGFA promoter in shnc and shATF5 cells by CHIP in hypoxia; p < 0.01. e Investigating the interaction between ATF5 and HIF1 transcriptional complex on endogenous VEGFA promoters by ChIP/Re-ChIP in hypoxia. f Whole-cell extracts of KYSE30 cells were collected for IP analysis using the indicated antibodies, followed by IB analysis in hypoxia. g Investigating which protein of HIF1 transcriptional complex directly binding to ATF5 by GST pull down. h Whole-cell extracts of shATF5 KYSE30 cells were collected for IP analysis using the indicated antibodies, followed by IB analysis in hypoxia