Fig. 3
From: ATF5 and HIF1α cooperatively activate HIF1 signaling pathway in esophageal cancer
Silencing of ATF5 inhibits the proliferation, migration, and invasion ability of ESCA cells. a Analysis of ATF5 expression level in shnc versus shATF5 cells by western blot. b Analysis of cell cycle in shnc versus shATF5 cells by PI assay. c Analysis of cell proliferation ability in shnc versus shATF5 cell by CCK8 assay; *p < 0.01. d Analysis of tumorigenicity in shnc versus shATF5 cells by colony formation assay; *p < 0.01. e Analysis of invasion and migration ability of shnc versus shATF5 cells by Transwell assay; Scale bar 100 μm(up) or 50 μm(down). f The migration ability of identified cells stably expressing shnc and shATF5 was analyzed through a wound healing assay; Scale bar 100 μm; p < 0.01