Fig. 2
From: ATF5 and HIF1α cooperatively activate HIF1 signaling pathway in esophageal cancer
ATF5 overexpression promotes the proliferation, migration, and invasion ability of ESCA cells. a Analysis of ATF5 expression level in vector versus ATF5 cells by western blot. b Analysis of cell cycle in vector versus ATF5 cells by PI assay. c Analysis of cell proliferation ability in vector versus ATF5 cells by CCK8 assay; *p < 0.01. d Analysis of tumorigenicity in vector versus ATF5 cells by colony formation assay; *p < 0.01. e The migration ability of identified cells stably expressing the empty vector or ATF5 was analyzed through a wound healing assay; Scale bar 100 μm; p < 0.01. f Analysis of invasion and migration ability in vector versus ATF5 cells by Transwell assay; Scale bar 100 μm (left) or 50 μm (right)