Fig. 2

Effects of D + Q treatment worsen liver disease progression. a Representative liver H&E images from control diet and DEN/HFD mice treated with D + Q and steatosis/ballooning score graph. b Distribution of tumor-bearing mice among the groups, with classification of dysplastic nodules based on the size in small nodules (diameter < 3 mm) and large nodules (diameter > 3 mm). c Representative liver β-gal staining + nuclear fast red (NFR) images of CTL, D + Q, DEN/HFD and HFD/DEN D + Q groups showing no difference in senescent positive cells. d Immunoblotting and qRT-PCR analysis of the senescence marker p16. *p < 0.05 Vs CTL determined by Student´s t test