Fig. 2

Analysis of activation of UPR and caspase pathway and UPR in GBM samples with different LAMP-2A expression. a We divided six GBM samples according to the LAMP-2A level as “High” expression group and “Low” expression group. The average LAMP-2A expression of the High group was about two times of that of the Low group. b UPR activation was examined by protein expression of p-PERK, p-IRE1, p-JNK and CHOP. Significant down-regulation of p-PERK, p-IRE1, p-JNK and CHOP was observed in the High group as compared with the Low group. c Activator proteins in apoptosis including caspase 3, caspase 9 and Bax, and anti-apoptotic protein Bcl-2 were measured by western blot. Caspase 3, caspase 9 and Bax expressions were inhibited while Bcl-2 expression was enhanced in the High group as compared with the Low group. d Apoptotic levels were examined by Tunnel analysis and compared within the two groups, with positive cells stained by orange. Patients with high LAMP-2A expression showed as half apoptosis rate as those with low LAMP-2A expression. The data are mean ± SEM from three glioblastoma samples. Protein levels are expressed relative to Low group set as 1. Significant changes are set as p < 0.05 and represented by asterisk (Two-tailed t test)