Fig. 1

Expressive analysis of LAMP-2A and N-CoR in clinical samples. a mRNA levels of LAMP-2A and N-CoR were measured by qRT-PCR. b Protein levels of LAMP-2A and N-CoR were measured by western blot. LAMP-2A mRNA and protein levels were significantly increased in GBM center (n = 8) in comparison with peri-tumor edema zone (PTEZ, n = 8) (p < 0.0001), while increasing trend was observed as compared with low grade glioma (LGG, n = 8). The protein level of N-CoR, but not mRNA level was significantly decreased in GBM center as compared with PTEZ (p < 0.0001). Linear regression analysis incorporating data from LGG, GBM center and PTEZ revealed moderate negative correlation between protein expression of LAMP-2A and that of N-CoR (r =  − 0.6001, p = 0.0019). c Immunohistochemistry (IHC) analysis of LAMP-2A and N-CoR (brown signal) in glioma clinical samples. Nucleus (blue signal) was stained with hematoxylin; D. immunofluorescence (IF) analysis of LAMP-2A (green signal) and N-CoR (red signal) in glioma clinical samples. DNA (blue signal) was stained with DAPI. Both IHC and IF studies displayed upregulation of LAMP-2A and downregulation of N-CoR in GBM centers. The data are mean ± SEM from 8 tissue specimens as a group. mRNA or protein levels are expressed relative to LGG set as 1. Significant changes are set as p < 0.05 and represented by asterisk (One-Way ANOVA; Bonferroni's test)