Fig. 6
From: Peptide targeting of lysophosphatidylinositol-sensing GPR55 for osteoclastogenesis tuning
LPI induces actin cytoskeleton remodelling through endogenous GPR55 in RAW264.7 cells. a FACS analysis dot plot of RAW264.7 cells (left) shows two main cell sub-populations (#1, #2) with different intrinsic features in both cell size and granularity, which were separately gated. Fluorescence distributions of #1 and #2 (right). Black, unstained cells; dark/light grey, unstimulated/LPI-stimulated phalloidin-stained RAW264.7 cells. b Phalloidin-stained wild-type RAW264.7 cells stimulated without or with 1 µM or 10 µM soybean LPI. Changes in mean fluorescences of the #1 and #2 cell sub-populations were recorded by FACS analysis. Data are mean fluorescences as percentages of unstimulated cell fluorescence, as means ± SE of at least five independent experiments. c Phalloidin-stained si-NT-interfered or si-GPR55-interfered RAW264.7 cells stimulated without and with 10 µM soybean LPI. Changes in mean fluorescences of the #1 and #2 cell sub-populations were recorded by FACS analysis. Data are mean fluorescences as percentages of unstimulated-si-NT-cell fluorescence, as means ± SE of five independent experiments. *p < 0.05 (Student’s t-tests)