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Fig. 11 | Cell Communication and Signaling

Fig. 11

From: Peptide targeting of lysophosphatidylinositol-sensing GPR55 for osteoclastogenesis tuning

Fig. 11

GPR55 modulators on osteoclast-resorbing activity. RAW264.7 cells were differentiated on bone slices with 15 ng/ml RANKL for 7 days in the presence of carriers or with the indicated compounds, and then the eroded surface was analysed (see “Methods”). a Representative image of resorption cavities, showing a trench (red arrow) and three pits (red asterisk). Scale bar, 50 µm. b Total resorption area on bone slice surfaces was quantified, and is here expressed as percent of the control (Ctrl) of RANKL-differentiated cells in the presence of 0.4% DMSO for the samples treated with 0.5 µM ML-191 or CID16020046, or with 0.004% fatty-acid free BSA for samples treated with 1 µM 18:0 LPI. Data are means ± SEM of at least three independent experiments performed at least in triplicate. c Trench cavities are expressed as the proportions (%) of the total resorption areas for each treatment. Data are means ± SEM of at least three independent experiments, performed at least in triplicate. *p < 0.05 versus Ctrl (Student’s t-tests). CID, CID16020046

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