Fig. 10
From: Peptide targeting of lysophosphatidylinositol-sensing GPR55 for osteoclastogenesis tuning
Peptide-P1 inhibits osteoclast maturation. Real-time PCR analysis of the differentiation markers (as indicated) in RAW264.7 cells treated without (–) or with 15 ng/mL RANKL for 72 h, in absence or in presence of 150 nM (0.2 µg/mL) Peptide-P1 (P1), or an irrelevant peptide (Irr_P, CGGNGPGLC). Transcripts were quantified and normalised using β2-microglobulin expression, as the housekeeping gene. Data are means ± SEM of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.005 versus RANKL (one-way ANOVA, followed by Fisher’s least significant difference tests)