Skip to main content
Fig. 5 | Cell Communication and Signaling

Fig. 5

From: The phospholipase D inhibitor FIPI potently blocks EGF-induced calcium signaling in human breast cancer cells

Fig. 5

Inhibition of Sig-1R also abrogates EGF-induced calcium release in MDA-NEO and MDA-HER2 cells. a MDA-NEO and MDA-HER2 breast cancer cells express Sig-1R. HeLa served as a positive control. Representative Western blot data from three independent experiments are shown. MDA-NEO cells (b) and MDA-HER2 cells (c) were pretreated with different concentrations of the PLD1 antagonist BD1047 and then stimulated with 100 ng/ml EGF. The results are presented as the mean ± S.E.M. of three independent experiments. Statistical analysis: one-way ANOVA and Tukey’s post hoc test: * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001

Back to article page