Fig. 4

EGF-induced calcium is significantly blocked after PLD1 knockdown. (a) qPCR data of relative PLD1 and PLD2 expression in PLD1 siRNA- and scRNA-transfected MDA-NEO and MDA-HER2 cells. The results are presented as the mean of two independent experiments. (b) Western blot data of immunoprecipitated PLD1 in scRNA- and siRNA-transfected MDA-NEO and MDA-HER2 cells. Representative blots of two independent experiments are shown. (c) Densitometric analysis of PLD1 IP/WB data. The results are presented as the mean ± S.E.M. of two independent experiments. Transfected MDA-NEO cells (d) and MDA-HER2 cells (e) were pretreated for 30 min with 100 nM FIPI and then stimulated with 100 ng/ml EGF. The results are presented as the mean ± S.E.M. of at least four independent experiments. Statistical analysis: one-way ANOVA and Tukey’s post hoc test: * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001