Fig. 6

mTOR is involved in CMTM7-induced autophagy. A HeLa cells were transfected with empty vector or CMTM7 plasmid, and after transfection for 24 h, the total and phosphorylation levels of mTOR and its downstream signaling molecules RPS6KB1 and 4E-BP1 were analyzed by Western blotting. B The total and phosphorylation levels of mTOR and its downstream signaling molecules RPS6KB1 and 4E-BP1 in CMTM7 knockdown and control cells underwent Western blotting. C CMTM7 knockdown and control cells were transfected with vector expressing GFP-tagged RHEB-D60K mutant for 24 h, and the cell extracts were analyzed by Western blotting as indicated. D CMTM7 knockdown and control cells were transfected with vector expressing GFP-tagged RHEB-D60K mutant for 24 h, and then treated with CQ (25 μM) for 4 h, and then the expression of LC3B was analyzed by Western blotting. The intensity of the bands for LC3B-II protein was analyzed by ImageJ software and normalized to the grey density of β-actin. The average relative grey density presented as s.d. from three independent experiments was shown in (E) (**P < 0.05, ***P < 0.001; ns, not significant)