Skip to main content
Fig. 5 | Cell Communication and Signaling

Fig. 5

From: CMTM7 as a novel molecule of ATG14L-Beclin1-VPS34 complex enhances autophagy by Rab5 to regulate tumorigenicity

Fig. 5

CMTM7 enhances the association of ATG14L-Beclin1-VPS34 complex with Rab5. A Control and CMTM7-myc-overexpressing HeLa cells were subjected to immunoprecipitation with anti-Rab5 antibody, anti-myc antibody or normal rabbit IgG, separated by SDS-PAGE and subjected to Western blotting. Immunoblots of the precipitates and the input were probed with the indicated antibodies. B Control and CMTM7-GFP-overexpressing HeLa cells were subjected to immunoprecipitation with anti-Rab5 antibody or normal rabbit IgG, separated by SDS-PAGE and then subjected to Western blotting. Immunoblots of the precipitates and the input were probed with the indicated antibodies. C Control and CMTM7-GFP-overexpressing HeLa cells were subjected to immunoprecipitation with anti-Beclin1 antibody, separated by SDS-PAGE and then subjected to Western blotting. Immunoblots of the precipitates and the input were probed with the indicated antibodies. D HeLa cells that were transiently transfected with CMTM7 were co-transfected with DN-Rab5. After 24 h post-transfection, the expression of LC3B was analyzed by Western blotting. β-actin was used as an internal standard. E CMTM7 knockdown and control cells were transfected with CA-Rab5. After post-transfection for 24 h, the expression of LC3B was analyzed by Western blotting. β-actin was used as an internal standard

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X