Fig. 6

Knockdown of PGRMC1 decreases the migratory ability of MDA-MB-231 cells. b Cell scratch assay analysis and quantification of MDA-MB-231 cells transfected with either Control siRNA or PGRMC1 siRNA (scale bar: 25 µm). b Transwell analysis and quantification of MDA-MB-231 cells transfected with either Control siRNA or PGRMC1 siRNA (scale bar: 50 µm). c Western blot analysis and quantification of PGRMC1, FAK, PCNA, ERα, PR, and HER2 in MDA-MB-231 cells transfected with either Control siRNA or PGRMC1 siRNA. GAPDH was used as an internal control. d mRNA expression of an epithelial marker (E-cadherin) and mesenchymal markers (N-cadherin, SLUG, VIM) in MDA-MB-231 cells transfected with either Control siRNA or PGRMC1 siRNA. Rplp0 was used as an internal control. Bars in the right panel show the mean ± standard deviation values. *p < 0.05 versus control siRNA