Fig. 2

Genetic deletion of Pgrmc1 does not suppress the growth and proliferation of breast cancer in MMTV-PyMT mice. a Gross images of tumor-bearing WT and Pgrmc1 KO mice at the age of 13 weeks. b Tumor weight (g) of WT and Pgrmc1 KO mice at the age of 13 weeks. c Tumor weight per body weight (BW) of WT and Pgrmc1 KO mice at the age of 13 weeks. d Immunostaining of Ki67 in the tumors of WT and Pgrmc1 KO mice. The number of Ki67-positive cells (pink) were counted. DAPI (blue) was used as an internal control. e Western blot analysis and quantification of PGRMC1, ERα, PR, HER2, PCNA, pAKT, pERK, PARP, and Cl. PARP. GAPDH was used as an internal control. Bar graphs in the right panel show the mean ± standard deviation values (WT, n = 10; KO, n = 16). *, p < 0.05 versus WT mice