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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: A new type of flexible CP12 protein in the marine diatom Thalassiosira pseudonana

Fig. 1

Western blot of CP12 expression. a Dark and light conditions. 20 µg crude extract from T. pseudonana cells grown under light (lane L) or dark (lane D). Molecular mass markers are shown on the right. After electrophoresis, the 12% SDS-gels are transferred onto nitrocellulose membranes and revealed with enhanced chemiluminescence with α- T. pseudonana CP12 antibodies 1:10,000. b Expression during the different phases of the growth curve. The growth of T. pseudonana in F/2 + Si medium, under continuous light and with air-concentration of CO2 (400 ppm) is followed by the absorbance at 680 nm. 30 μg of proteins from day 1 to 8 are loaded on SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and revealed with antibodies raised against α- T. pseudonana CP12 antibodies

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