Fig. 3

Regulation of uPA activity affected the acceleration of skin wound healing induced by SPD treatment. a–e Multiple independent excisional biopsies (5 mm diameter) were taken from the right and left dorsum of each mouse. a, b To inhibit uPA activity, amiloride (10 mg/kg) was intraperitoneally administered to the mice every day after wound creation. The rates of wound closure among the following four groups were compared: the SPD untreated group, SPD untreated + amiloride group, SPD systemic administration group and SPD systemic administration + amiloride group. c–e To inhibit PAI-1 function, MDI-2268 was administered intraperitoneally (3 mg/kg) to mice for 2 consecutive days after wounding. The rates of wound closure among the following six groups were compared: the SPD untreated group, SPD untreated + MDI-2268 group, SPD topical administration group, SPD topical administration + MDI-2268 group, SPD systemic administration group, and SPD systemic administration + MDI-2268 group. The mean rate of wound repair was calculated based on the original wound area of each biopsy site. *Indicates a statistically significant differences; p < 0.05. **Indicates a statistically significant difference; p < 0.01. ***Indicates a statistically significant difference; p < 0.005