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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: The epithelial-mesenchymal transition and the cytoskeleton in bioengineered systems

Fig. 5

EMT and vimentin in confined spaces. a Micropillar arrays for confined migration fabricated using an elastomeric silicone with 10 \(\upmu\)m spacing and height. b Mammary epithelial cells (MCF-10A) migrate as individuals breaking away from a collective front after EMT induction via the Snail transcription factor. Immunofluorescence staining of the nucleus (blue), E-cadherin (green), and vimentin (red). Reproduced from [97]. c Transwell (Boyden Chamber) assay for migration through confined pores. d Nuclear morphology of wild type and vimentin knockout fibroblasts after traversing membrane. Immunofluorescnece staining of the nucleus (blue), Lamin-A (green), and Lamin B (red). Reproduced from [98]

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